abstract：:The level of 1,25(OH)2D3 receptors in cultured mouse osteoblast-like (OB) cells is modulated by the rate of cell proliferation. We have studied two 1,25(OH)2D3-induced bioresponses to ascertain whether the changes in receptor levels during growth in culture alter cell responsiveness. Nuclear receptor levels were high ...

journal_title：Journal of cellular physiology

authors： Chen TL,Li JM,Ye TV,Cone CM,Feldman D

更新日期：1986-01-01 00:00:00

abstract：:We recently derived a series of transformed cell lines by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with a newly described human gene, R-myc, which has homology to the c-myc oncogene. In this report, we show that these lines share some features characteristic of cells of the monon...

journal_title：Journal of cellular physiology

authors： Sklar MD,Tereba A,Chen BD,Walker WS

更新日期：1985-12-01 00:00:00

abstract：:A neuroblastoma cell line was assessed for its capacity to bind tetanus toxin (TT) by using immunofluorescence and flow cytometry to analyze cells on a single cell basis. A clone of Neuro 2a, N2AB-1, was shown to bind variable amounts of TT per cell and this binding could be saturated by increasing doses of the toxin....

journal_title：Journal of cellular physiology

authors： Notter MF,Leary JF

更新日期：1985-12-01 00:00:00

abstract：:The smooth muscle cell population in major arteries of humans and experimental animals is heterogeneous with regard to cellular DNA content. A proportion of cells has polyploid DNA content and this proportion increases with normal aging and with hypertension. We have isolated pure populations of rat aortic smooth musc...

journal_title：Journal of cellular physiology

authors： Rosen EM,Goldberg ID,Shapiro HM,Zoller LC,Myrick KV,Levenson SE,Halpin PA

更新日期：1985-12-01 00:00:00

abstract：:High potassium concentrations altered the morphology and the ability to grow in soft agar in 6m2 cells, a clone of rat kidney cells infected with a temperature-sensitive mutant of Moloney sarcoma virus. Approximately 60% of cells exhibited normal morphology in the presence of 94.8 mM potassium in isotonic medium at th...

journal_title：Journal of cellular physiology

authors： Lai CN,Becker FF

更新日期：1985-11-01 00:00:00

abstract：:We investigated the actions of human placental lactogen (HPL) and human growth hormone (HGH) on [3H]thymidine incorporation and the release of immunoassayable somatomedin-C (SM-C) by isolated myoblasts, dermal fibroblasts, and costal cartilage explants taken from human fetuses at 11-21 weeks of gestation. The incorpor...

journal_title：Journal of cellular physiology

authors： Hill DJ,Crace CJ,Milner RD

更新日期：1985-11-01 00:00:00

abstract：:rac-1-O-Myristoyl-2-O-acetylglycerol, rac-1-O-palmitoyl-2-O-acetylglycerol, and rac-1-O-oleoyl-2-O-acetylglycerol acted like phorbol myristate acetate and mezerein in stimulating human neutrophil aggregation. Responses to these agents were equally influenced by cytochalasin B, extracellular calcium and magnesium, arac...

journal_title：Journal of cellular physiology

authors： O'Flaherty JT,Schmitt JD,Wykle RL,Redman JF Jr,McCall CE

更新日期：1985-11-01 00:00:00

abstract：:Neurite formation and proliferation by NG108-15 cells were studied in short-term serum-free N2 medium. Neuritogenesis by individual cells was observed at widely differing times, suggesting a stochastic component to initiation of differentiation. Cells with and without neurites could also enter one or more rounds of pr...

journal_title：Journal of cellular physiology

authors： Krystosek A

更新日期：1985-11-01 00:00:00

abstract：:Using rapid kinetic techniques, we have determined the kinetics of zero-trans influx and equilibrium exchange of adenosine, and its uptake and in situ phosphorylation at 25 degrees C in human erythrocytes which were pretreated with 2'-deoxycoformycin to inhibit deamination of adenosine. Both the Km and Vmax for adenos...

journal_title：Journal of cellular physiology

authors： Plagemann PG,Wohlhueter RM,Kraupp M

更新日期：1985-11-01 00:00:00

abstract：:gamma-Glutamyl transpeptidase has multi-catalytic activities. It degrades glutathione and can produce ammonia from glutamine. The present study was designed to examine whether the decreased cell proliferation, cellular glutathione content and concurrent increase in ammonia production in senescent cells in culture are ...

journal_title：Journal of cellular physiology

authors： Takahashi S,Nakagawa S,Zeydel M,Bhargava G

更新日期：1985-10-01 00:00:00

abstract：:The effects of adenosine and two analogs, L-phenylisopropyladenosine (L-PIA) and 5'-N-ethylcarboxamidoadenosine (NECA), on cAMP production and on platelet-derived growth factor (PDGF)-stimulated initiation of DNA synthesis in growth-arrested cultures of rat arterial smooth muscle cells (SMC) were studied. The intracel...

journal_title：Journal of cellular physiology

authors： Jonzon B,Nilsson J,Fredholm BB

更新日期：1985-09-01 00:00:00

abstract：:We have previously reported that the proliferation of cloned MCF-7 and T47D human mammary tumor cells can be inhibited by increasing concentrations of charcoal-dextran stripped female human serum (CDFHS). The maximal proliferation rate was restored by the addition of 3 X 10(-11) M estradiol-17 beta to the culture medi...

journal_title：Journal of cellular physiology

authors： Schatz RW,Soto AM,Sonnenschein C

更新日期：1985-09-01 00:00:00

abstract：:A linear pathway of morphologically intermediate cells has been identified between the cuboidal epithelial stem cells and the doming alveolar-like cultures of the cell line Rat Mammary (Rama) 25 in the order: cuboidal----grey----dark----dark droplet cell----doming cultures. The overall process can be accelerated by di...

journal_title：Journal of cellular physiology

authors： Paterson FC,Rudland PS

更新日期：1985-09-01 00:00:00

abstract：:Differentiation of 3T3-L1 adipocytes, monitored by accumulation of neutral lipid and by increase in alpha-glycerophosphate dehydrogenase activity, is accelerated by incubation of confluent 3T3-L1 fibroblasts in media containing insulin, dexamethasone and isobutylmethylxantine (IBMX). IBMX inhibits cyclic nucleotide ph...

journal_title：Journal of cellular physiology

authors： Elks ML,Manganiello VC

更新日期：1985-08-01 00:00:00

abstract：:Studies on the receptor specificity and dynamics involved in fibroblast phagocytosis of latex beads revealed the following: 1) Ligands other than fibronectin such as concanavalin A (ConA) and serum spreading factor, when coated on latex beads, were found to promote phagocytosis of the beads. This indicates that fibrob...

journal_title：Journal of cellular physiology

authors： McAbee DD,Grinnell F

更新日期：1985-08-01 00:00:00

abstract：:A factor, termed neutrophil alkaline phosphatase-inducing factor (NAP-IF), that has the capacity to increase the NAP activity of granulocytes was characterized by using two samples: cystic fluid (CF) and conditioned medium of a tumor cell line (T3M5). The molecular weight of NAP-IF was shown to be between 13,000 and 4...

journal_title：Journal of cellular physiology

authors： Sato N,Asano S,Mori M,Ueyama Y,Sugano K,Ohsawa N,Takaku F

更新日期：1985-08-01 00:00:00

abstract：:In fertilized echinoderm eggs, the male and female pronuclei fuse to form the diploid nucleus even in the presence of aphidicolin, a specific inhibitor of eukaryotic DNA polymerase-alpha. The subsequent first cleavage is independent of chromosomes but dependent on spindle and amphiaster. The fate of DNA originally exi...

journal_title：Journal of cellular physiology

authors： Yamada H,Hirai S,Ikegami S,Kawarada Y,Okuhara E,Nagano H

更新日期：1985-07-01 00:00:00

abstract：:It is well known that cell proliferation (and hence, DNA synthesis) declines in human diploid fibroblast-like cells with increasing passage number. It is not clear whether DNA synthesis declines in the remaining cells that are still actively proliferating. Estimations of cell kinetic parameters permitted extrapolation...

journal_title：Journal of cellular physiology

authors： Collins JM,Chu AK

更新日期：1985-07-01 00:00:00

abstract：:Shortly after serum-deprived BALB/c 3T3 fibroblasts are stimulated to grow in medium containing 10% calf serum, the RNA polymerase I activity in permeabilized cells shows a two-fold increase over the values observed in either serum-deprived or density-inhibited resting cells. Inhibition of protein synthesis by pactamy...

journal_title：Journal of cellular physiology

authors： Perrone-Bizzozero N,Iapalucci-Espinoza S,Medrano EE,Franze-Fernández MT

更新日期：1985-07-01 00:00:00

abstract：:Cultured bovine aortic endothelial cells were fixed and stained with NBD-phallicidin and quantitated with a digital image analyzer for changes in actin cables and surface area. Serotonin (5-HT), norepinephrine (NE), dopamine and histamine (all at 10(-4)M concentrations) were tested for their ability to induce cytoskel...

journal_title：Journal of cellular physiology

authors： Welles SL,Shepro D,Hechtman HB

更新日期：1985-06-01 00:00:00

abstract：:Optical indicators of the cationic, cyanine and anionic oxonol classes were used to evaluate the plasma membrane potential of animal cells in suspension and in monolayer culture. The optical signals were calibrated by using diffusion potentials either of K+ (in the presence of valinomycin) or of H+ (in the presence of...

journal_title：Journal of cellular physiology

authors： Bashford CL,Alder GM,Gray MA,Micklem KJ,Taylor CC,Turek PJ,Pasternak CA

更新日期：1985-06-01 00:00:00

abstract：:We have previously isolated 3T3 cell variants unable to respond to specific mitogens. In this report we analyze the dominant and/or recessive nature of these variants. Two independently isolated EGF nonproliferative variants are unable to bind EGF. Hybrids between 3T3R5 cells (thymidine kinase deficient, ouabain-resis...

journal_title：Journal of cellular physiology

authors： Terwilliger E,Herschman HR

更新日期：1985-06-01 00:00:00

abstract：:Four temperature-sensitive (ts) mutants of rat 3Y1 fibroblasts, representing independent complementation groups, cease to proliferate predominantly with a 2n DNA content, at the restrictive temperature (39.8 degrees C) (temperature arrest) or at the permissive temperature (33.8 degrees C) at a confluent cell density (...

journal_title：Journal of cellular physiology

authors： Mitsudomi T,Kimura G

更新日期：1985-06-01 00:00:00

abstract：:The present study extends our previous observation (Kasambalides and Lanks, J. Cell. Physiol., 114:93-98, 1983), that dexamethasone inhibits the alterations in heat shock protein (HSP) and glucose-regulated protein (GRP) synthesis caused by glucose deprivation. We now show that insulin, even in the presence of high ex...

journal_title：Journal of cellular physiology

authors： Kasambalides EJ,Lanks KW

更新日期：1985-05-01 00:00:00

abstract：:Cultured bovine adrenocortical cells were previously shown to be functionally deficient in selenium and vitamin E when grown in medium supplemented with fetal bovine serum. In the present experiments, the lack of significant bioavailable amounts of selenium in the medium was demonstrated by the finding of only low lev...

journal_title：Journal of cellular physiology

authors： Hornsby PJ,Pearson DW,Autor AP,Aldern KA,Harris SE

更新日期：1985-04-01 00:00:00

abstract：:Cell hybrids were formed between human diploid fibroblasts (HDF) and carcinogen-transformed HDF to determine the relationship among: (1) finite proliferative lifespan, which we define as an age-related failure of a population to achieve one population doubling in 4 weeks; (2) arrest in a senescent state, which we defi...

journal_title：Journal of cellular physiology

authors： Stein GH,Namba M,Corsaro CM

更新日期：1985-03-01 00:00:00

abstract：:We compared the effects of 95% O2 (hyperoxia) alone, endotoxin (20 ng/ml) alone, and 95% O2 plus endotoxin on the release of lactate dehydrogenase (LDH), uptake of 5-hydroxytryptamine (5-HT), and antioxidant enzyme activities in porcine pulmonary arterial and aortic endothelial cells in monolayer culture. Hyperoxia in...

journal_title：Journal of cellular physiology

authors： Block ER,Patel JM,Sheridan NP

更新日期：1985-02-01 00:00:00

abstract：:CSF-1 is a hemopoietic growth factor that specifically regulates the survival, proliferation, and differentiation of mononuclear phagocytic cells. Populations of adherent bone marrow-derived macrophages (BMM) devoid of CSF-1 producing cells were used to study regulation by CSF-1 of macrophage entry into S phase. More ...

journal_title：Journal of cellular physiology

authors： Tushinski RJ,Stanley ER

更新日期：1985-02-01 00:00:00

abstract：:Previous studies suggested that the decreased collagen production observed in Kirsten sarcoma virus (Ki-MSV)-transformed BALB 3T3 cells could be reversed by treating cells with Bt2cAMP. We examined the relationship between intracellular cAMP, collagen production, and other properties in NRK and BALB 3T3 cells transfor...

journal_title：Journal of cellular physiology

authors： Majmudar G,Peterkofsky B

更新日期：1985-01-01 00:00:00

abstract：:Splenic erythroblasts obtained from mice during the acute disease caused by either the polycythemia-inducing (FVP) or anemia-inducing (FVA) strain of Friend virus were examined for their degree of terminal differentiation. Morphology, benzidine staining, and heme synthesis kinetics showed that many erythroblasts from ...

journal_title：Journal of cellular physiology

authors： Koury MJ,Sawyer ST,Bondurant MC

更新日期：1984-12-01 00:00:00

abstract：:C6 glioma cells possess beta adrenergic receptors coupled with adenylate cyclase which can be irreversibly blocked by bromoacetylaminomethylpindolol (Br-AAM-pindolol), a beta adrenergic antagonist. With 1 microM Br-AAM-pindolol, more than 80% of beta adrenergic receptors, labeled by (3H)-dihydroalprenolol [3H)-DHA), w...

journal_title：Journal of cellular physiology

authors： Homburger V,Pantaloni C,Lucas M,Gozlan H,Bockaert J

更新日期：1984-12-01 00:00:00

abstract：:The growth factors FGF and vasopressin were found to have only a transient effect on confluent quiescent monolayers of Swiss 3T3 cells. Whether measured as cumulative entry into S-phase by autoradiography, or as cell division by time-lapse filming, the elevated rate of cell proliferation was maintained only over 10-15...

journal_title：Journal of cellular physiology

authors： Richmond KM,Riddle PN,Brooks RF

更新日期：1984-12-01 00:00:00

abstract：:The effect of cell culture age and concomitant changes in cell density on the biosynthesis of sulfated-proteoglycan by rabbit articular chondrocytes in secondary monolayer culture was studied. Low density (LD, 2 d), middle density (MD, 5-7 d), and high density (HD, 12-15 d) cultures demonstrated changes in cellular mo...

journal_title：Journal of cellular physiology

authors： Malemud CJ,Papay RS

更新日期：1984-12-01 00:00:00

abstract：:The frequencies of 6-thioguanine-resistant primary clones from the kidneys and skeletal muscles of aging male cohorts of two F1 hybrid strains of Mus musculus varied from 0.59 to 10.96 X 10(-5) and did not increase as a function of donor age (up to 40 months). Resistant clones were shown to be severely deficient in th...

journal_title：Journal of cellular physiology

authors： Horn PL,Turker MS,Ogburn CE,Disteche CM,Martin GM

更新日期：1984-11-01 00:00:00

abstract：:Kinetic studies of binding and internalization of 125I-platelet-derived growth factor (PDGF) demonstrate that up to 15% of membrane-associated radioactivity is internalized within 2 minutes after warming to 37 degrees C in a variety of cell types. The T 1/2 for internalization is approximately 20 minutes. The T 1/2 fo...

journal_title：Journal of cellular physiology

authors： Rosenfeld ME,Bowen-Pope DF,Ross R

更新日期：1984-11-01 00:00:00

abstract：:Endothelial cell-derived growth factor (ECDGF) is a soluble mitogen secreted in vitro by bovine aortic endothelium. ECDGF is a mixture of at least two distinct heat-stable and trypsin-sensitive mitogens. Large amounts of mitogenic activity were found in lysates prepared from cultured endothelial cells. Other nonmitoge...

journal_title：Journal of cellular physiology

authors： Gajdusek CM,Schwartz SM

更新日期：1984-11-01 00:00:00

abstract：:The cells responsible for the long-term in vitro generation of murine mast cells have been examined. Sequential analysis of all colony types obtained from cultures of spleen or bone marrow cells showed that only colonies derived from multipotential cells (mixed-erythroid colonies) or mast cell progenitors, contained c...

journal_title：Journal of cellular physiology

authors： Li CL,Johnson GR

更新日期：1984-10-01 00:00:00

abstract：:Several hydrolase activities characteristic of the apical brush border membrane of renal proximal tubule, leucine aminopeptidase, gamma-glutamyl transpeptidase, alkaline phosphatase, maltase, and trehalase, were identified in cultures of the LLC-PK1 kidney epithelial cell line. A coordinate increase in activities of t...

journal_title：Journal of cellular physiology

authors： Yoneyama Y,Lever JE

更新日期：1984-10-01 00:00:00

abstract：:Experiments were conducted to determine the extent and variability of collagen degradation in human fetal lung fibroblasts. Cells were incubated with [14C]proline, and degradation was measured by determining the hydroxy[14C]proline in a low molecular weight fraction relative to total hydroxy[14C]proline. Average (basa...

journal_title：Journal of cellular physiology

authors： Bienkowski RS

更新日期：1984-10-01 00:00:00

abstract：:Stimulation of amiloride-sensitive sodium (Na+) influx and the subsequent activation of NA+, K+-ATPase by serum or growth factors have been implicated as early events leading to initiation of cell proliferation. We recently demonstrated that amiloride inhibits thrombin-initiated DNA synthesis not by inhibiting an earl...

journal_title：Journal of cellular physiology

authors： Stiernberg J,Carney DH,Fenton JW 2nd,LaBelle EF

更新日期：1984-09-01 00:00:00